منابع مشابه
The Mechanism of the Rhodanese-catalyzed Thiosulf atekipoate Reaction
The individual reactions comprising the rhodanesecatalyzed thiosulfate-lipoate reaction were demonstrated kinetically. The mechanism was shown to be a reaction sequence in which the enzyme forms kinetically significant binary complexes with both thiosulfate and dihydrolipoate substrates. Moreover, the first product (sulfite) is discharged, forming a sulfur-substituted enzyme intermediate, befor...
متن کاملThe rhodanese/Cdc25 phosphatase superfamily
Rhodanese domains are ubiquitous structural modules occurring in the three major evolutionary phyla. They are found as tandem repeats, with the C-terminal domain hosting the properly structured active-site Cys residue, as single domain proteins or in combination with distinct protein domains. An increasing number of reports indicate that rhodanese modules are versatile sulfur carriers that have...
متن کاملReversible Folding of Rhodanese
For the first time completely reversible unfolding was achieved for guanidinium chloride-denatured rhodanese using a systematically defined protocol. These conditions included fl-mercaptoethanol, lauryl maltoside, and sodium thiosulfate. All components were required to get more than the previous best reactivation with lauryl maltoside of 17% (Tandon, S., and Horowitz, P. (1986) J. Biol. Chem. 2...
متن کاملCrystalline beef kidney rhodanese.
Although rhodanese activity occurs in many tissues (l-3), the enzyme had been obtained in crystalline form from beef liver only (4). Beef kidney rhodanese could not be crystallized by the same procedure (5). To extend the biochemical genetic comparison reported previously (5), however, it was desired to obtain the kidney enzyme in a very high state of purity. This paper reports the purification...
متن کاملStudies on the active site of rhodanese.
The complete loss of enzymic activity on reaction with alkylating agents, aromatic nitro compounds, or aliphatic mercaptans indicates the importance of sulfhydryl groups for catalysis. Analyses during the course of inactivation with any of these reagents revealed the loss of one of the two -SH groups in the rhodanese monomer when inactivation was complete. Amino acid analysis of iodoacetate-ina...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1966
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)96474-4